I don't know why this gif makes me chuckle like it does. It could be solely due to the fact that Eli Manning is the principle actor. It could be his exasperation and animated motions when he realizes when things don't go his way. It could just be the end, when the ball goes flying by him as the playclock winds down.
In fact, it's probably just the whole package. Sad and frustrated Eli Manning is the best.
You can't stop watching this. It's almost hypnotizing.