Zdenek Zeman has opted for a strong trident upfront, and finally plays Daniele De Rossi in his favoured central midfield position.
Goalkeepers Maarten Stekelenburg and Bogdan Lobont are injured, so Mauro Goicoechea is thrust into his first derby between the sticks.
Elsewhere, Mattia Destro is suspended after picking up two quick bookings during last weekend's win over Palermo.
AS Roma: Mauro Goicoechea; Ivan Piris, Marquinhos, Nicolas Burdisso, Federico Balzaretti; Michael Bradley, Daniele De Rossi, Alessandro Florenzi; Erik Lamela, Pablo Daniel Osvaldo, Francesco Totti
Subs (from) Tomas Svedkauskas, Rodrigo Taddei, Dodò, Leandro Castan, Alessio Romagnoli, Panagiotis Tachtsidis, Simone Perrotta, Marquinho, Miralem Pjanic, Nico Lopez